DECAL: Deoxyribozyme-Catalyzed Labeling of RNA
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منابع مشابه
Deoxyribozyme-catalyzed labeling of RNA.
Site-specific covalent modification of RNA is important for enabling structure–function studies. For example, probes such as fluorescein are commonly used in fluorescence resonant energy transfer (FRET) investigations of RNA folding. Biotin is used for immobilization during single-molecule analysis, to enable RNA–protein cross-linking studies, and as a key element of selection schemes in vitro....
متن کاملUse of deoxyribozymes in RNA research.
Since their first identification by in vitro selection in 1994, deoxyribozymes have been developed to catalyze a variety of chemical reactions. The first DNA-catalyzed reaction was cleavage of a ribonucleotide linkage within an oligonucleotide substrate. In subsequent years, growing collections of deoxyribozymes have been developed for several reactions that have practical utility for RNA resea...
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The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2',5'-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were f...
متن کاملKinetic and thermodynamic characterization of the RNA-cleaving 8-17 deoxyribozyme.
The 8-17 deoxyribozyme is a small DNA catalyst of significant applicative interest. We have analyzed the kinetic features of a well behaved 8-17 construct and determined the influence of several reaction conditions on such features, providing a basis for further exploration of the deoxyribozyme mechanism. The 8-17 bound its substrate with a rate constant approximately 10-fold lower than those t...
متن کاملDNA-catalyzed formation of nucleopeptide linkages.
Covalent linkages between nucleic acids and amino acid side chains are important in many biological contexts. For example, phosphodiester bonds between the 5’-end of DNA or RNA and the side chain of tyrosine are formed during topoisomerase activity and in genomes of picornaviruses, such as poliovirus, and an RNA 5’-terminus is linked to a serine side chain of the tumor-suppressor protein p53. I...
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تاریخ انتشار 2007